Why Native PAGE gel is empty?

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Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Subjects number in SEM for experimental design?

For an experimental design ( one experiment and one control group), at least or minimum, how many subjects need to be included in the groups in SEM?

08 January 2024 8,210 3 View

Article Removal Request?

07 November 2023 4,567 2 View

How can i turn any survey result into mathematical modelling ?

What I want to ask is this: I have a survey and its analysis. I want to build a mathematical model using these analyses. how can I do it

04 September 2023 9,331 3 View

Why do we use sodium cyanide in this protocol of sod assay? What happen if i don't use?

Superoxide Dismutase Assay Method Activity of SOD has been determined in two ways: 1. Inhibition by the enzyme of an O2- dependent reaction. 2. Pulse radiolytic methods (Rigo et al. 1975) See:...

14 August 2023 3,191 1 View

If X predicts Y, and Y predicts Z; does that reflect an indirect relationship?

Hello dear researchers In my study, I have 5 independent variables and 1 dependent variable. In multiple regression analysis, not all my IVs significantly predicted my DV. However, most of my IVs...

27 October 2022 8,623 4 View

Has anyone used siRNA from Synbio Technologies before?

Hello, I am planning to do gene knockdown using siRNA. Has anyone used siRNA from Synbio Technologies before? If so, what are your thoughts?

19 March 2022 4,655 0 View

How can I transform effect sizes (cohen's d) before running a meta-analysis in R?

I'm using R to run a meta-analysis (using Robust Variance Estimation) of Cohen's d effect sizes. What transformations do I need to do and how do I do them in R? Does anyone have access to online...

13 September 2021 7,239 3 View

What is the optimal candidal biofilm adhesion time to determine at the candida albicans biofilm inhibiting effect of a substance?

candida albicans biofilm

22 May 2021 9,688 0 View

What is the optimal candidal biofilm adhesion time to determine at the candidal biofilm inhibiting effect of a substance?

candidal biofilm

20 May 2021 4,792 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View