I want to infect my triple negative breast cancer cell line with the Adeno-associated virus. Is that possible to infect my cell line with any Adeno-associated virus serotype? Thank you.
Hi Muhammed,
Because you are working with cell line, I think you don't need an AAV with a specific tissue-tropism. But maybe you should use a serotype more commonly used, like serotype 2 or 9.
Good luck
Dear Muhammed,
the two following methods (a chemical one and a physical one) will allow you enhancing infection/transduction in permissive cells or to infect/transduce non permissive cells. They are compatible with any AAV and adenoV serotype and also with any cell type.
- AdenoMag is based on the Magnetofection technology***. It uses magnetic beads to concentrate adenoviral particles onto the cell surface within minutes. as an example it was shown to be efficient with AAV (https://repositories.tdl.org/uh-ir/handle/10657/ETD-UH-2012-05-418).
- AdenoBlast is based on an adenovirus binding peptide that assists transduction by coupling the adenoviral particles to the cell membrane in a CAR independent manner.
These two reagents are very efficient,
would you like to have more information, please do not hesitate to contact me directly at: [email protected] or via researchgate
good luck in your experiment,
best regards,
Cedric
*** The Magnetofection technology uses a magnetic field to attract and concentrate complexes of magnetic nanoparticles and nucleic acid onto the cell surface as demonstrated by Grześkowiak BF et al, Pharm Res. 2014 Jul 18. (http://www.ncbi.nlm.nih.gov/pubmed/25033763).
http://www.ozbiosciences.com/transduction-infection/29-adenomag-magnetofection-reagent-adenovirus-transduction.html
http://www.ozbiosciences.com/transduction-infection/74-adenoblast-transduction-infection-adenovirus-booster.html
Try AAV-DJ, AAV-1, or AAV-6. Those are almost always the best in cell culture, particularly AAV-DJ. AAV-8 is great in vivo but usually very poor in culture.
However, if you're using a cell line then AAV won't be much good. It doesn't integrate, so it will disappear from your culture as your cells divide.
Jill Muhling, thank you so much for sharing your help and experience on this matter.
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