I want to check my zymolase is working or not. So, It need to check its activity in spheroblast formation. So I need a proper protocol with prescribed concentration of zymolase.
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
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01 August 2024 711 1 View
"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...
29 July 2024 2,181 7 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...
21 July 2024 8,807 6 View
I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??
19 July 2024 8,571 1 View
Could you provide information on the average energy consumption per gate operation when using superconducting qubits, specifically focusing on the microwave power required to implement gates? I...
15 July 2024 954 0 View
I've found that some journals are both Scopus-indexed and listed on Beall's list as predatory or potentially predatory. Why does this discrepancy occur? Are there any more reliable platforms than...
12 July 2024 5,158 1 View
I have been trying to delete one copy of an essential gene from a diploid in fission yeast. I am using PCR based homologous recombination method and primers are designed from Bahler's lab website....
12 May 2024 1,274 4 View
I am adding enough adenine in my plates
06 October 2023 365 5 View
Please share the protocols for transformation in diploid strain of fission yeast
06 July 2023 6,941 0 View
I was tring to check the differential binding/association of my protein to genes using mutant S. pombe. For this, I used a Flag tagged normal strain and a Flag tagged mutant strain. I did...
11 January 2022 7,271 0 View
I was expecting a similar kind of effect when I overexpress my protein of interest in a temperature sensitive mutant in permissible temperature as it is bound to behave like wt cells but I...
16 November 2021 6,622 2 View
Hi fission yeast researchers, I made a batch of 1M hydroxyurea solution last year and stored in -20 freezer. I wonder if I can still use them now? Or do I need to make fresh ones? Thank you! Best...
17 June 2021 7,032 0 View
I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...
18 September 2020 7,789 2 View
Has anyone has any issues with their synthetic minimal media dropout plates only supporting growth for a week or two after being poured? Our lab is having this problem for both S. cerevisiae and...
16 June 2019 4,441 8 View
I have been using this protocol to extract dna from fission yeast though it is not working. I am getting two bands that are very small in size. Can someone troubleshoot with me...
07 August 2018 1,939 0 View
One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?
05 June 2016 8,239 3 View