How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to Draw Crystal Structure from powder XRD?

Hi, I would appreciate if anyone can recommend on the process of drawing crystal structure from observed powder xrd diffractogram and if there is any suggested software to work on.

05 January 2021 3,517 3 View

What does this PZC data mean?

I measured different kinds of catalysts which were dispersed in DI water.I firstly added certain amount of HCl and then used NaOH to titrate and measure the pH value. What does this result mean?

27 December 2020 5,728 4 View

Why mass flowrates of atoms at the inlet and outlet of a reactor are not conserved (CFD with chemical reactions, species model - eddy dissipation)?

Hi, guys. Thanks for reading this question. I am modeling a reactor (steady, RANS). CH3OH and O2 go into the reactor via inlet (a jet). After reactions, species leave the computational domain...

08 December 2020 7,362 13 View

How to analyze a time series of temperature mean whether different or not?

I have 6 culture tank, 2 treatment with 3 replicate. I test temperature of every tank everyday, for 14 days. Now I want to compare whether this 2 treatment temperature have significant different...

06 December 2020 834 7 View

Is the pH value will affect the calculated overpotential in OER process by DFT?

27 November 2020 1,456 2 View

How to transform this implicit FDTD iteration equation into the matrix equation?

11 October 2020 9,042 1 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View

Can anyone help to determine concentration of hydroxyurea necessary to culture K562 cell line to investigate some genes expression?

I want to know how to calculate the concentration of hydroxyurea and the cell count in 24-well plate.

10 November 2018 8,958 1 View

Extraction of Chromosomal DNA from Schizosaccharomyces pombe?

I have been using this protocol to extract dna from fission yeast though it is not working. I am getting two bands that are very small in size. Can someone troubleshoot with me...

07 August 2018 1,890 0 View

What is the best protocol to follow when performing scratch assay for over-expression or drug studies?

I perform scratch assay by over-expressing or adding drugs, like doxorubicin or hydroxyurea. I looked for various protocols online, but there is no clear procedure as to how long the transfection...

10 November 2017 1,180 5 View

What will be a suitable dose of hydroxyurea in tumor bearing mice?

I am currently trying to study tumor regression in mice xenograft models using treatment with hydroxyurea. There seem to be limited publications and I am trying hard to determine a suitable dose...

09 November 2016 1,838 3 View

If one of the proteins in S.pombe was tagged with FLAG, is this pombe still considered as wild type?

One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?

05 June 2016 8,096 3 View

If one of the proteins in S.pombe was tagged with FLAG, is this pombe still considered as wild type?

One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?

01 June 2016 5,020 3 View

How to perform a deletion mutation in S Pombe ?

I tried to perform a deletion mutation in fission yeast using the 2 step PCR method. The target gene should be replaced by KanMX6.Can I have a reliable and detailed protocol?Thank you in advance...

01 February 2016 7,277 3 View

Could someone give me insight into why gene duplications could be occurring when targeting essential genes in S. pombe?

We are trying to make conditional mutants in essential genes of Schizosaccharomyces pombe, using the nmt1 promoter and different degrons, and antibiotic selection in haploid cells. The homologous...

21 October 2015 1,638 3 View

Has anyone tried to replicate or confirm cell-cycle dependent gene expression and failed ([email protected])?

Sometimes people try to replicate a published result such as gene expression variation during the cell cycle and fail, and thus do not publish as this is a "negative" result and journals generally...

04 May 2015 6,638 0 View