I have been using this protocol to extract dna from fission yeast though it is not working. I am getting two bands that are very small in size. Can someone troubleshoot with me ?
http://cshprotocols.cshlp.org/content/2016/5/pdb.prot090985.full
I have 3 inserts one is 464 bp, 1173 bp and 713 bp. I am trying to ligate and amplify them using pcr. I have done a overnight ligation and found very faint bands on my gel. I try to amplify though...
31 December 2017 715 0 View
I am wanting to fuse 3 dna together and insert them in a plasmid. Is fusion pcr?
11 December 2017 1,653 0 View
I am trying to fuze 3 different inserts together. I am looking for a good fusion protocol.
11 December 2017 3,646 2 View
I am looking for a protocol using 3 dna pcr.
11 December 2017 4,569 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View