Could anyone suggest me a good molecular marker for primary screening of Alternaria species?

04 June 2015 1 6K Report

Because of the having large number of Alternaria isolates and lack of adequate molecular tools i am searching for an appropriate molecular marker to differentiate Alternaria species and then i will identified the selected isolates

Bilal A. Padder

Since Alternaria Spp are difficult to characterize morphologically, the best approach will be use of combinations of few genes like ITS, 1EF, btublin etc. Amplify genes using primers and later on sequence them. Use softwares like Paup, mega etc for phylogeny. By this way u will be able to differentiate different species of fungus. One marker can't help in this case, if u would have been dealing with oomycets then ITS is enough.

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