Glucose assay standard preparation/getting purple color, what is causing this?

Does anybody have the experience of getting a purple color for standards in Glucose assay? after adding sulphuric acid, the color turns to purple rather than pink. What do you think is causing...

31 December 2016 2,415 1 View

Does anyone have any idea how to inhibit liquid loss inside the capillary tube?

I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...

08 September 2015 3,712 8 View

Can anyone help me with some problems I am having with primer Tm?

I have a set of primers designed by IDT and the Tm for most of them is 62 but I am using Quant fast SYBR green which has an annealing temperature of 60 C and says that the primers should have the...

05 June 2014 9,404 5 View

Which one is recommended: Treating RNA with DNAase, or treating the cDNA with an RNAase inhibitor?

I want to make cDNA from RNA extractions and I am wondering if it needs to be treated with RNAase or DNAase?

04 May 2014 441 21 View

Is EDTA a must in DNase treatment after RNA extraction?

I have some frozen RNA samples in -80 and I want to do cDNA synthesis. I have a protocol for DNase treatment with DNase I from bio lab as follows: DNAse mix DNAse 1.0...

04 May 2014 480 31 View

Does anyone know what the ideal proportions of cDNA and primer are for preparing master mix in qPCR?

I've got so many recipes and I am confused which one is best. However, I tried one which apparently did not work and I could just see the amplification for my cyclophillin not for target genes. I...

03 April 2014 5,153 8 View

Which one to trust: RNA or cDNA concentration?

I am doing qPCR based on some samples which have been done by another student. My confusion is that I have some nanodrop for RNA extracted samples and also the nanodrop for cDNA samples...

03 April 2014 601 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View