Saeideh Davoodi

8 Questions 11 Answers 0 Followers

Questions related from Saeideh Davoodi

Could anyone suggest a good way to dissolve lard for making a high fat diet?

I am trying to make a ketogenic diet using lard and wonder how to dissolve lard and have a solidify mix of carbohydrate+protein+ lard at the end for 1 litre?

05 May 2017 3,108 4 View

Glucose assay standard preparation/getting purple color, what is causing this?

Does anybody have the experience of getting a purple color for standards in Glucose assay? after adding sulphuric acid, the color turns to purple rather than pink. What do you think is causing...

01 January 2017 2,460 1 View

Does anyone have any idea how to inhibit liquid loss inside the capillary tube?

I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...

09 September 2015 3,748 8 View

Can anyone help me with some problems I am having with primer Tm?

I have a set of primers designed by IDT and the Tm for most of them is 62 but I am using Quant fast SYBR green which has an annealing temperature of 60 C and says that the primers should have the...

06 June 2014 9,448 5 View

Is EDTA a must in DNase treatment after RNA extraction?

I have some frozen RNA samples in -80 and I want to do cDNA synthesis. I have a protocol for DNase treatment with DNase I from bio lab as follows: DNAse mix DNAse 1.0...

05 May 2014 525 31 View

Which one is recommended: Treating RNA with DNAase, or treating the cDNA with an RNAase inhibitor?

I want to make cDNA from RNA extractions and I am wondering if it needs to be treated with RNAase or DNAase?

05 May 2014 484 21 View

Does anyone know what the ideal proportions of cDNA and primer are for preparing master mix in qPCR?

I've got so many recipes and I am confused which one is best. However, I tried one which apparently did not work and I could just see the amplification for my cyclophillin not for target genes. I...

04 April 2014 5,195 8 View

Which one to trust: RNA or cDNA concentration?

I am doing qPCR based on some samples which have been done by another student. My confusion is that I have some nanodrop for RNA extracted samples and also the nanodrop for cDNA samples...

04 April 2014 645 5 View