I am specifically looking at methods of antibody conjugation to minimize loss of antibody and also developing a stable system to be used in lateral flow assay.
There are functionalized iron oxide nanoparticles for sale. If you choose to use carboxylic acid functionalized NP, you can conjugate your ab through EDC/NHS linking. You can start with 0.1M EDC/0.2M NHS in MES buffer pH 5.0 for activating NP. Then you react your ab with the activated iron oxide nanoparticles for 2 hours. You need to test for conjugation efficiency and the best ab/NP ratio by using UV spectrometry.