Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

22 October 2020 9,855 3 View

What are the peaks at 14.3 and 28.4 in LDH's XRD pattern?

Hi! I'm synthesizing MgAl-NO3-, MgAl-Cl-, MgAl-CO32-, etc. by coprecipitation method using metal nitrate salts, NaOH, and anion source coming from sodium salts. Besides those characteristic peaks...

21 September 2020 3,402 3 View

LDH Cytotoxicity Assay for NK92 cells, high background?

I'm trying to optimize an LDH cytotoxicity assay (https://www.takarabio.com/products/cell-biology-assays/cell-biology-reagents/ldh-cytotoxicity) but am running into some issues. I'm using NK92s as...

03 February 2020 6,502 1 View

Cytotoxicity assay from culture supernatant?

Hi, I was planning to perform a cytotoxcity assay wherein I am planning to use the culture supernatant stored frozen at -80 deg C at different time points. Is there any suggestion with regards to...

18 November 2019 3,724 1 View

What is this 'cloud' that appears around my cell aggregates during drug toxicity tests?

I am performing tests by adding increasing concentrations of drugs to my cell culture media (using DMSO as a vehicle), but in the higher concentrations especially I see these large dark cloud...

11 September 2019 6,419 4 View

What is this 'cloud' that appears around my cell aggregates during drug toxicity tests?

I am performing tests by adding increasing concentrations of drugs to my cell culture media (using DMSO as a vehicle), but in the higher concentrations especially I see these large dark cloud...

08 September 2019 3,560 5 View

Nitrogen adsorption desorption experiment BET to find surface area and pore diameter?

i want to find out surface area and pore diameter of LDH by nitrogen adsorption desorption experiment. i am usnig my sample in mg (low quantity) for dye removal. how i can recover my sample after...

05 June 2019 8,653 4 View

Which metals can bring novality in structure of Layered double hydroxide to increase its adsorption effeciency for dyes?

I am working on dyes removal from aqueous solution by using LDH. already there are alot of LDHs used regarding to this application. I want to bring novality by changing the structure and...

02 March 2019 1,504 8 View

Does any one know why in an LDH with M-N-X chemical formula, the M2+ and N3+ ions dissolve differently in aging condition? L/S and pH effect?

during aging of my LDH material, Ca and Al ions dissolve with different proportions in aging process. At pH 8, L/S 20 Ca dissolves more than Al. However, at pH 12, L/S 20 Al dissolved more....

17 January 2019 159 5 View

Does anyone have any idea why my Nuclear Westerns Blots would look like this?

I am attempting to measure nuclear and cytosolic proteins from human muscle. Currently, I am having trouble with my nuclear western blots. As you can see from the attached image The first and...

31 December 2018 3,717 3 View