Jyoti Kumbhar

5 Questions 8 Answers 0 Followers

Questions related from Jyoti Kumbhar

Could anyone please provide a protocol for conjugation of antibodies to iron oxide nanoparticles?

I am specifically looking at methods of antibody conjugation to minimize loss of antibody and also developing a stable system to be used in lateral flow assay.

03 October 2019 7,166 2 View

How do I conjugate a biotinylated peptide with streptavidin conjugated gold nanoparticle? Is there a specific protocol?

Gold nanoparticles were synthesized by citrate reduction method (Size=20nm). The peptide is 17 amino acid residue long with a biotin molecule attached to a lysine residue the carboxy-terminus. The...

18 February 2015 3,610 0 View

How can I detach cells from a scaffold used for tissue engineering applications?

I am working on hydrogel like scaffolds and trying to detach cells after incubation for 2, 4, etc days. But the cells do not come out in suspension, hence it is difficult for me to quantitate cell...

08 January 2014 7,617 7 View

How to enhance cell seeding on scaffolds and minimize cell attachment onto the bottom of a tissue culture plate?

During cell culture on scaffolds, most of the cells migrate to the bottom and attach to the bottom of the tissue culture plate. How can one prevent this? I have read about a method which calls for...

18 December 2013 3,670 8 View

How to obtain a standard curve for mineralization by osteoblasts, with the Alizarin red S method?

I am able to get a standard curve for higher than 2mM concentrations using the CPC method, but not for lower concentrations. In addition, I am unable to get a standard curve using acetic acid...

10 October 2013 2,138 11 View