Hello everyone, I need to evaluate leptin levels in the serum of rats in vivo treated with silica nanoparticles. I have difficulties in finding robust commercial ELISA assays to evaluate this and other adipokines (for instance, I tried to measure adiponectin by commercial kits providing pre-coated plates, but it was impossible to have results since the standard curve did not work). Do you have any suggestion based on your experimental experience?
Thanks in advance.