How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Is there any chance of a person getting reinfected by COVID-19 Virus?

Is there any available data that shows reinfection by COVID-19 Virus? What is the immune status against COVID-19 virus of an affected person, once he/she gets cured?

01 June 2020 190 12 View

Is simulation technology has any role in laparoscopic surgical training ?

Minimally invasive surgery technique has taken the central role in modern surgery specially in abdominal surgery. So, every surgeons need to get trained into the field of minimally invasive...

25 May 2020 6,412 9 View

What will be the best way to concentrate very low molecular weight proteins or peptides?

Hi everyone, I am looking for any recommendation on concentrating very low molecular weight proteins (~1-3 kD). I would appreciate if anyone has experience in dealing with that low protein size....

10 September 2019 3,853 4 View

Is there any parameter file for GTP or GMP containing nucleic acid in AMBER or NAMD?

How to get the parameter file for GTP or GMP containing nucleic acid in AMBER or NAMD?

20 July 2019 9,501 3 View

How does abaqus calculate principal stress?

I am modelling a uniacial compression test of cylinder in abaqus. I need to extract principal stress value.I extracted force on surface, divided by area to get engineering stress value. After...

17 June 2018 5,843 14 View

Design of constant pressure gas chamber?

Any suggestions on how to design a glass chamber inside which the oxygen gas will be at a constant pressure of 1-1.5 bar throughout the experiments. Thanks

28 November 2017 4,976 3 View

Is there any other method to calculate EDLC and pseudocapacitance contribution of a supercapacitor material other than Trasatti method?

Trasatti and Power's law are used to calculate the edlc and the pseudocapacitive contribution of the electrode material. But I am facing a problem to calculate via Power's Law. In power's Law a...

17 October 2017 2,571 5 View

At what circumstances in a Donor-Acceptor type molecular system 1CT (charge transfer) emission will be dominant over 1LE (localized exciton) emission?

Generally in D-A (donor-acceptor) based TADF system, it has been shown the CT emission are dominant most of the time than their individual 1LE donor or 1LE acceptor emission. This is happen when...

18 January 2017 1,200 5 View

What is the solvent for Liraglutide API ?

09 July 2015 597 5 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View