Gabriella Di Felice

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Questions related from Gabriella Di Felice

Could anyone help me in selecting a good commercial ELISA assay to evaluate leptin in rat serum?

Hello everyone, I need to evaluate leptin levels in the serum of rats in vivo treated with silica nanoparticles. I have difficulties in finding robust commercial ELISA assays to evaluate this and...

12 October 2015 2,519 11 View

Which is the best method to solubilize formazan crystals after MTT cell labeling in proliferation assays?

I'm setting up a MTT assay to evaluate cell proliferation and cytotoxicity of mouse spleen cells after polyclonal and antigen-specific stimulation in 96-well flat-bottomed plates. After 4 hour MTT...

16 December 2013 3,395 32 View

Freezing BMDC at the end of the differentiation culture in GM-CSF (day 8-10): do you have experience about any effect on BMDC maturation and function?

We prepare mouse bone marrow-derived DC according to the protocol of Lutz (J Imm Meth 223,1999:77–92). I am in particular interested in CD86 expression and inflammatory cytokine production

06 March 2013 7,050 1 View