Vincent Paul Schmitz

3 Questions 79 Answers 0 Followers

Questions related from Vincent Paul Schmitz

Using a Strong Anion Exchange (SAX) HPLC Column to Purify a Small (Negatively Charged) Protein?

Does anyone have any experience with this? I have a mixture of five proteins, the one I want purified out is ~10 kDa with a pI ~4.5. I have a strong anion exchanger: 150mm x 4.6mm, 4um, 80A pore...

04 April 2019 6,563 3 View

For G-Quadruplex Formation, Would any Combination of (G3+)(N1-7)(G3+)(N1-7)(G3+)(N1-7)(G3+) Cause a Quadruplex? What Values are Optimal?

I've read that the following formula: (G3+)(N1-7)(G3+)(N1-7)(G3+)(N1-7)(G3+) is used to algorithmically search for potential g-quadruplex motifs in various genomes. I'm wondering whether certain...

03 March 2019 6,912 2 View

Ethanol, Isopropanol, or Bleach - Which one is the best for sensitive PCR?

At each of the labs I've been in they used one of the aforementioned solutions to sterilize and clean the work area, tools, containers, etc. The lab I have just joined insists on using 10% bleach...

02 February 2019 5,372 6 View