Could anybody give me a protocol on how to isolate protein from paraffin embedded tissue blocks?

10 April 2014 1 1K Report

Regarding protein isolation.

Dear Rim, depends on what you want to do with it. In case you are looking for protein identification/quantitation by mass spectrometry, look up publications by Wisniewski and Mann using the FASP protocol. There is even a video tutorial available. For the intact proteins, I do not know.

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