I am doing research on storage strawberry. I want to find out N-linked glycans from storage strawberry fruits. Please provide me suitable protocol. Thanks in advance.
need to know protocol for photoplate creation, styles, etc
11 July 2024 8,321 2 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View
Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...
25 June 2024 9,151 6 View
Any Upcoming call for papers in journals (SCI/SCIE indexed) related to image processing or computer vision. Special issues related to my area, whose submission dates are within a few months.
23 June 2024 5,021 1 View
I want to know the suitable etchant required to reveal the Aluminum oxide grain boundaries.
14 June 2024 8,955 1 View
Apart from XRD analysis, I want to know the other techniques used for identifying the oxide phases (even when a particular oxide is having very less volume fraction) present in a thermally grown...
14 June 2024 3,555 5 View
pI of my protein is 4.1. I am using Ni coumn to purify it . Which binding buffers will be good? Even the pI is low can it still bind to nucleic acid? How do I know?
04 May 2024 8,535 2 View
It is a feedback question for Reserach purpose.
02 April 2024 3,136 0 View
I'm using Jurkat E6.1 cell line, kindly recommend what media should I use; at present I have RPMI-1640 without sod pyruvate & 2g/L glucose, is it ok?
17 March 2024 4,747 3 View
I want to interpret saed pattern of hrtem. For that i want to compare my value of radius of ring with standard Jcpds card. where i would get to interpret hkl values?
21 January 2024 9,891 0 View
The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...
01 August 2024 3,519 4 View
Hi everyone, this topic is completely new to me, so i need some help to proceed with the easiest (if possible) method to achieve proteins extraction from strawberry fruits! Thanks in...
22 April 2024 2,609 0 View
I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...
21 February 2024 7,127 1 View
Which is the most sweetest variety of strawberry in Indian condition.
06 January 2024 1,839 1 View
Hi all, For the imaging of fine structure on the surface of spike proteins, I want to try to cleave glycans on the protein surface. Is there any enzyme/method that can be used to do it without...
27 October 2023 3,798 0 View
Hi all, I used pngase f to release glycan from transferrin. here is my protocol. 1. I prepared storage buffer for the pngase f without glycerol and aliquot them into different vial 2. I took 10...
21 October 2023 3,658 7 View
The genotyping data we have comes from a GBS analysis, I added screenshot from our data as example. We also have our GBS data available in a VCF file. The phenotypical is scoring data from a Bio...
17 October 2023 8,974 0 View
Dear all, I used both mass photometry and SEC-MALS to define the mass of my recombinant protein. Based on the amino acid sequence only, it should be 92 kDa. However, it holds different glycan...
27 July 2023 4,339 2 View
I am doing the antioxidant enzyme activity assay in strawberries but by testing various protocols in articles still couldn't reach any acceptable results. Can you tell me a suitable protocol or...
29 May 2023 7,030 0 View
I am using GLYCAM in AMBER to conduct simulations of glycans. But the system I am working on contains modified monosaccharide residues GlcNAc6S. Basically, there is a sulfate group attached to C6...
20 May 2023 5,347 1 View