Coomassie Staining of gels/membranes: Methanol or Ethanol?

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Fish sold (A) by traders versus fish bought (B) by consumers at Market X?

I have a case in my field research I want to know the consistency of the fish troops at Market X I recorded data for 7 days (from August 11 to August 17) regarding: (A) Fish sold by traders at...

27 September 2023 9,526 0 View

Ikan yang dijual (A) oleh pedagang versus ikan yang dibeli (B) oleh konsumen di Pasar X?

Saya mempunya sebuah kasus dalam penelitian lapangan saya Saya ingin mengetahui konsistensi pasukan ikan di Pasar X Saya mendata selama 7 hari (dari tanggal 11 Agustus hingga tanggal 17 Agustus)...

27 September 2023 9,295 0 View

Very low cell yield when using Sony SH 800 sorter?

Hello! I use a Sony SH 800 sorter to sort differentiated tumor cells (CD44-CD24+) of the T47D line. According to our and literature data, the CD44-CD24+ population in the T47D line is 70-80%,...

04 December 2022 988 2 View

What regulatory documents, international standards and forums have information about hot taps (hot-tapping) and how to use them safely?

My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...

10 January 2021 7,053 3 View

Does anybody have a UK injury statistics and environmental damage from gas pipelines accidents?

I need statistics on "Accidents at hazardous production facilities (gas pipelines, oil pipelines, boiler houses, coal mines and other enterprises that use hazardous substances)", and also as...

30 April 2020 7,470 0 View

While measuring GCaMP6 responce to light from photoreceptor cells, how can we ensure there is no overlap with the stimulation light signal??

I'm currently reading papers about in vivo retina activity measurement with calcium indicators and I can not understand, why the retinal photoreceptor cells do not activate in responce to the...

06 April 2020 7,771 0 View

Does anyone have access to the database of the images from classical Hodgkin lymphoma?

Images from classic Hodgkin lymphoma, H&E, CD15, CD30, PAX5

11 December 2019 1,609 2 View

All inorganic perovskite solar cell?

Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...

05 July 2019 5,815 7 View

Explain different mechanism of 2D layer perovskite and 3D perovksite layer?

I am working on perovskite solar cell, i want to prepare 2D perovskite layer by adding Pb(SCN)2 in active layer

07 July 2018 2,525 9 View

Please any one help me, I need numerical data of fires (coordinares and any information apart from images) for Europe?

Coordinates,AOD, Angstrom parameter or smth.

21 January 2015 2,939 5 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View