What regulatory documents, international standards and forums have information about hot taps (hot-tapping) and how to use them safely?

My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...

10 January 2021 6,815 3 View

Coomassie Staining of gels/membranes: Methanol or Ethanol?

Manufacturers and protocols recommend using Methanol, but in our lab they use Ethanol instead. Is there any difference? I need to stain PVDF membrane.

04 September 2020 9,855 3 View

Does anyone have access to the database of the images from classical Hodgkin lymphoma?

Images from classic Hodgkin lymphoma, H&E, CD15, CD30, PAX5

11 December 2019 1,494 2 View

All inorganic perovskite solar cell?

Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...

05 July 2019 5,695 7 View

2X RNA Loading and ladder

2X RNA Loading and ladder? I have to dilute 2X RNA loading dye with water. Usually I use 3 micro liter of loading with 3 micro liter of my sample. How much water should I use for diluting 2X RNA...

27 November 2011 520 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What are damage parameters for SMA while modelling on ABAQUS 2020?

I have given Elastic and Superelastic material properties, but I think we need to provide the damage parameters also. Please help me here.

16 February 2021 8,694 1 View