What regulatory documents, international standards and forums have information about hot taps (hot-tapping) and how to use them safely?

My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...

10 January 2021 6,815 3 View

Coomassie Staining of gels/membranes: Methanol or Ethanol?

Manufacturers and protocols recommend using Methanol, but in our lab they use Ethanol instead. Is there any difference? I need to stain PVDF membrane.

04 September 2020 9,855 3 View

Does anyone have access to the database of the images from classical Hodgkin lymphoma?

Images from classic Hodgkin lymphoma, H&E, CD15, CD30, PAX5

11 December 2019 1,494 2 View

All inorganic perovskite solar cell?

Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...

05 July 2019 5,695 7 View

2X RNA Loading and ladder

2X RNA Loading and ladder? I have to dilute 2X RNA loading dye with water. Usually I use 3 micro liter of loading with 3 micro liter of my sample. How much water should I use for diluting 2X RNA...

27 November 2011 520 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View