Hi,
I have assayed the lipolytic activity of lactic acid bacteria isolated from olives by spectrophotometric method. I used lipase from porcine (Sigma) to generate a standard curve. I tested bacteria but I can't convert the results from microgram to Units.
The lipase box says following:
Lipase from porcine pancreas
Type II, 100-500 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
So, 1 microgram/miligram = How many Units? I couldn't calculate this. Please help.
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