Seda Tht

4 Questions 12 Answers 0 Followers

Questions related from Seda Tht

Convert to Units in Lipolytic activity assay?

Hi,I have assayed the lipolytic activity of lactic acid bacteria isolated from olives by spectrophotometric method. I used lipase from porcine (Sigma) to generate a standard curve. I tested...

10 October 2015 2,464 6 View

What is the problem of lipolytic activity assay?

Hi, I prepared the medium by adding peptone, NaCl, CaCl2•2H2O, agar and Tween-20 for lipolytic activity assay. And cultures are spotted into the medium. Finally I hoped clear zones around...

05 May 2015 699 10 View

Preparation standard curve for lipolytic activity?

I need to prepare standard curve using tributyrin as substrate for a lipase activity assay. I don't know I must use how much enzyme (mg or g). Can you help me?

05 May 2015 1,425 3 View

How is cetrimide agar used for pectinolytic activity ?

I used MP-5 medium and cetrimide agar for pectinolytic activity. for this firstly, I prepared MP5 medium and spotted on plates from cultures, after incubation added %1 cetrimide agar. Finally I...

05 May 2015 7,102 6 View