- Dear researchers, please help us to sort it out regarding one tricky conversion, In our lab, we are handling with DNaseI solution(1mg/ml). In one of the phage DNA isolation protocol mentioning 10 micro litres of 20 units DNaseI. but we have a product with 1mg/ml. how to convert mg/ml into units.
http://himedialabs.com/TD/ML068.pdf
You need to know how many units of activity you DNase I prep has per unit of mass (ie per ug or mg). If you have commercial DNase, it should provide the activity per mass on the label or instruction sheet.
Hello Ksds Roopa Kumar
I agree with Michael. You should visit the company's website and try to get information on the specific activity of DNase I. Once you know the u/mg value of DNase I you can calculate the concentration of DNase I solution in terms of u/ml. Let me give you an example. Suppose the specific activity of DNase I is 3u/mg and the label on the vial says 5mg/ml. Then the concentration of DNase I solution will be 15 u/ml.
Thank you.
I agree with Michael but I would also add that maybe you want to check the definition of unit in your specific protocol, since they can change depending on the manufacture.
For example:
-This one has a unit definition which correlate with 0.3 Kunitz (https://www.thermofisher.com/order/catalog/product/89836#/89836)
-However, this one use exactly the definition of Kunitz (https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Roche/Bulletin/1/11284932001bul.pdf)
Therefore, you need to determine both, the units per mg and the unit definition of your protocol.
Thank you David Rincón Fernández-Pacheco , Michael J. Benedik for the valuable suggestions
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