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Questions related from Ksds Roopa Kumar
We are dealing with 3 different primers which target 3 different genes of particular plant virus. The actual product size is about 422, 420, 380 bp and it was doing well for 2 years. But results...
11 April 2021 4,703 4 View
for example, we designed a new primer pair which is specific to coat protein of a particular plant virus. and its melting temperature is recorded as follows: Forward: 62.2 C Reverse: 60.6 C So, in...
06 April 2021 7,857 3 View
For example, I need to characterize Rhizoctonia and Trichoderma simultaneously. In literature they are giving ITS1 &ITS3 for Trichoderma and ITS2 &ITS4 for Rhizoctonia. In our lab there is...
20 January 2021 4,252 3 View
Dear researchers, please help us to sort it out regarding one tricky conversion, In our lab, we are handling with DNaseI solution(1mg/ml). In one of the phage DNA isolation protocol mentioning 10...
01 December 2020 7,504 4 View
