I want to know a protein that can be used as a control with molecular weight in the range 100-180 kda
Hi, depends what you want to do, but what about a bovine IgG standard? Christian
Hi, it will depend on you purpose and the protein you want to investigate as Christian Schmelzer said.
I am trying nucleolar isolation from hela cells.
05 June 2014 3,700 2 View
I am using T4 DNA ligase V: I ratio I tried is 1 to 3 but I could not get any clone.
05 June 2014 3,931 5 View
I have isolated total RNA from u2os (human) cell line. All times my 28 RNA band was not sharp from 18s RNA it got degraded. Anyone have suggestions?
10 November 2012 786 2 View
Is the brca 1 loss of heterozygosity is required for cancer, if yes then why it's inheritance is dominant ?
09 October 2011 8,941 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
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Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View