Is the brca 1 loss of heterozygosity is required for cancer, if yes then why it's inheritance is dominant ?
I am trying nucleolar isolation from hela cells.
05 June 2014 3,700 2 View
I am using T4 DNA ligase V: I ratio I tried is 1 to 3 but I could not get any clone.
05 June 2014 3,931 5 View
I have isolated total RNA from u2os (human) cell line. All times my 28 RNA band was not sharp from 18s RNA it got degraded. Anyone have suggestions?
10 November 2012 786 2 View
I want to know a protein that can be used as a control with molecular weight in the range 100-180 kda
10 November 2012 4,270 2 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...
10 June 2024 7,606 3 View
Describe the principles of Population Genetics and evolution as applied to livestock breeding programs
08 June 2024 2,924 0 View
I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...
29 May 2024 6,911 0 View
Hi, I've recently come across the colony of Geothrichum silvicola (based on sequencing result) isolated from soil with slimy morphology, unlike the colony morphology I've found on the journal...
13 May 2024 1,356 8 View
Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...
07 May 2024 5,119 3 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
Who (first) proposed/used/coined the term ‘translation’ in biology/genetics? What is the history behind the use of the word? Thank you!
01 May 2024 4,528 10 View
Can anyone assist with running the Linkage Analysis Tool 'Easylinkage' or any alternative tool for conducting linkage analysis and calculating LOD scores?
06 April 2024 7,273 2 View