Can anyone give me the name of a good company that I can order/buy human and rat cDNA. I will be using this as a + control. Preferably a pool. I would like to use this for qPCR.
I'm working on library prep for ITS NGS using Earth Microbiome Protocol and am getting double banding and smearing on my gels. What might be the cause for this? I should be seeing a band around...
04 March 2024 5,940 1 View
I am about to calculate alpha-amylase activity for some fungal samples. I followed the protocol of Sigma-Aldrich...
11 April 2022 3,763 9 View
Hi All, I am wondering how long are unused tissue slides, used for IHC, last at 4 degrees? I see conflicting reports in the literature. I have 1 yr. old tissue slides stores at 4 degrees. Am I...
19 July 2021 5,956 5 View
Hi there. I am performing some life imaging experiments and would like to look at the nucleus using either Dapi or Hoechst. I am wondering if anyone could share a protocol. My understanding is...
07 June 2021 6,261 3 View
Is it possible to transduce a human CAR into mouse T cells and have the mouse T cells function when the CAR binds it's antigen? I am thinking that if anything the singling domain of the CAR, as it...
10 May 2021 6,131 2 View
Many papers say they recovered the initial BPB (S)-2-[N-(N′-benzylprolyl)amino]benzophenone ligand after getting their amino acid from the Ni (II) Schiff base complexation, but I can't find...
19 April 2021 3,297 1 View
Hello, I am somewhat new to dynamic light scattering. I am working with a heterogeneous mixture of carbon based particles synthesized at various hydrogen peroxide concentrations. It's worth noting...
12 July 2020 905 2 View
From the literature we've seen research mostly examines pregnancy in adults (>20 years), but our population is 14-18 years old. We'd love any feedback on if we should go with cutpoints for...
09 June 2020 3,064 1 View
I'm trying to identify the species of even genus of this bacteria I have grown in the lab but due to its unique colony appearance the bacteria I am getting from decision trees are not showing any...
25 November 2018 5,040 0 View
I've been working with bEnd.3 and sEnd.1 cells which are endothelial cell lines from the periphery (sEnd1.s) and the brain (bEnd.1s). They are immortalised using middle T antigen. I need to serum...
22 March 2018 9,297 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View