Is it possible to transduce a human CAR into mouse T cells and have the mouse T cells function when the CAR binds it's antigen? I am thinking that if anything the singling domain of the CAR, as it is human, may be a problem for the mouse T cells.
Hi Bethany,
Sorry to respond to an old thread -- but couldnt help myself!
This is what our lab is trying to do at the moment. It is a very interesting thought and gives lots of possibilities to new mouse models for testing CAR T cell treatments. Based on previous literature I would say the answer is yes. This paper (Human CD19-Targeted Mouse T Cells Induce B Cell Aplasia and Toxicity in Human CD19 Transgenic Mice - PubMed (nih.gov) 10.1016/j.ymthe.2018.04.006) puts a CAR with human endodomains into mouse T cells and found that the hybrid CAR T cells are functional in vitro.
Hope that helps!
All the best,
Oliver
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