The effect of 0.05% Sodium azide on RNA extraction and quantification?

Dear RG users, I am dealing with a cervical sample collected with brush and placed in a specimen transport medium (contains 0.05% sodium azide) for HPV detection and genotyping. I have also a...

23 March 2020 1,107 3 View

How viruses evolved?

I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...

04 March 2018 2,871 4 View

What are the different types of cancer vaccines currently being in use?

30 November 2017 6,056 3 View

Is quantitative data analysis is the most accurate type?

People think numbers are more accurate than words although the quality of data analysis matters. Which one do you thin is more convenient in applied research? What are the...

15 November 2017 6,182 5 View

Conserved regions of HPV genome?

07 July 2017 766 3 View

Rapid diagnostic (point-of-care) options for cervical cancer associated with HPV?

29 June 2017 2,917 30 View

Any article exploring weather rabies could be transmitted with contamination of open wound with saliva from rabid animal?

08 May 2017 3,263 3 View

Transformation of African Agriculture

If you were asked to make top three priorities to transform African agriculture, what would you propose?. List only top 3 critical interventions to be addressed.

01 January 1970 6,325 14 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

What is the best bioinformatic tool to predict the interaction of peptide with bacterial cell wall membrane?

Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...

01 February 2021 1,351 3 View

Are any database collected cell penetrating anticancer peptides ?

Dear researchers, Anticancer peptides(ACPs) with cell-penetrating properties can enter to cytoplasmic space of cancer cells and induce cell death. but, I could not find the database that...

29 January 2021 707 3 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View

What is the best antimicrobial agent among metal oxides?

I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?

21 January 2021 7,068 5 View