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Information literacy in higher education integration with curricula engineering
04 August 2024 5,368 3 View
Please scientific articles on methodologies to create dashboards in R shiny and PowerBI
27 May 2024 387 1 View
Referring to the Article about challenges facing blood transfusion services in western Kenya, the question has come to mind as it is within the scope of my interest and in hopes that the answers...
10 April 2024 2,305 0 View
Hi everyone, I hope someone could help me. I have two bacterial strains that I isolated by streaking them in solid media and collecting an individual colony, repeating this process 2-3 times to...
01 April 2024 6,866 3 View
I work in a investigation about hydrolysis of protein of a andean grain and is very important for continue with my work what method is more effective for the activation or maybe is possible not to...
10 March 2024 6,629 2 View
Inquire about trends in training for new researchers Thank you for contributing
10 August 2023 7,233 3 View
Semiconductors Cu wire bonding susceptibility of Cu-Al IMCs to corrosion ? I am doing a research to understand what elements need to be present to have galvanic corrosion effect
21 June 2023 3,920 2 View
Our final project for a college course involves the expansion of PC-12 Adh cells. We have been using DMEM's culture medium supplemented with L-Glutamine, FBS and Pen/Strep antibiotics. The cells...
13 May 2023 3,171 2 View
Currently, the RGPPM technique is being focused on measuring the carbon footprint during the delivery of merchandise in last-mile logistics. However, it is important to establish KPIs related to...
12 May 2023 5,264 2 View
Describe some macro and micro techniques to identify mycoplasma presence in cell culture.
11 May 2023 9,089 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,105 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,850 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
