I am working on a project looking at the cytotoxic effects of lipid oxidation products on caco-2 cells. I would like to include a sample group with added antioxidants as a 'negative control'. What commercially available antioxidants might be suitable or have been used before? What concentrations are generally used? Are there other considerations to keep in mind? Thanks!
"Butylated hydroxytoluene, also known as dibutylhydroxytoluene, is a lipophilic organic compound, chemically a derivative of phenol, that is useful for its antioxidant properties." from Wikipedia. I would not use the concentration > (1-10) mM.
DTT, ascorbate, vitamin E, vitamin D, vanillin, citrulline, glutathione, salicylic acid and various indoles can be used.
In general ascorbate is probably the most commonly used due to low toxicity even at molar doses.
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