What pH buffer solution best replicates physiological conditions?

I am studying the interaction between protein (HSA) and liposomes (made with DMPC) and I would like to study under physiological pH for application in studying protein interaction with DDS. I have...

10 November 2020 3,594 3 View

All teacher educators are under the lockdown since March, what could they be doing, how is this lockdown going to affect tutors' performance ?

There are many COVID 19 related issues in terms of how to deal with the continuum of teaching and learning. I am meditating about what the future performance results will be for both the student...

16 June 2020 199 3 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

What is an acceptable sampling rate for skin conductance?

What is an acceptable sampling rate for measuring skin conductance? Research seems to use various rates, from 32Hz to 1000Hz. Is there a minimum rate advised? I have a device that measures at 4Hz...

03 February 2019 9,630 1 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

26 January 2018 2,096 3 View

How to calculate the Feed Conversion Rate (FCR) in free-choice diets?

Hi! I would like to calculate the FCR for a diet-induced obesity experiment in rats. Since I have many nutrients/groups, I am calculating the food intake (FI) in kcal, not in grams of food. Has...

09 November 2017 5,258 5 View

What is the typical percentage error when corneal endothelial cells are counted using a specular microscopy ?

11 July 2016 7,906 2 View

How do I design Methylight primer/probes without using Beacon?

I want to Methylight assays and I need to be able to design the primer/probe sets, or get software that can do it for me. But I've only been able to find Beacon software that does both primers and...

08 June 2016 9,414 1 View

What Australian cities would be good to compare with Geelong, Australia in regard to economic, social & physical capital?

Newcastle and Wollongong are good examples but I am interested to learn about other large regional centres, particularly those that may have experienced a shift in their economy and have had to...

26 November 2015 5,759 3 View

Which type of cell cultures are used in cosmetic industries to test treatments?

09 November 2015 5,890 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Which statistical test is best to seperate out treatment effects meta-analysis?

I am doing a meta-analysis and am looking for some guidance on which statistical test will help me do the following. I want to understand the effect size a treatment has on a population. There...

16 February 2021 8,033 3 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

Resume Book UEP2013 Asian Edition, dis somebodey has it?

I am looking for a copy of the resume book of UEP2013 Asian Edition (Urban Environmental Pollution 2013 Asian Edition) held in Beijing. If somebody has it, please I would like to obtain an...

11 February 2021 2,247 2 View