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Questions related from Fiona Burmeister Mccracken
I am planning an experiment where I stimulate ex vivo PBMCs in plates for seven days before analysis of the T cell proliferation. This appears to be a pretty standard experiment, however, I can't...
28 September 2023 8,702 2 View
Does anyone have any papers characterizing CD5bright CD8+ CD4- T cells? I'm working with canine peripheral blood samples and noticed a distinct population but I can't find any discussion in the...
07 September 2023 8,607 0 View
Is it plausible that cells exposed to an oxidative stressor would display increased metabolism (as measured through MTT assays)?
26 June 2023 7,002 2 View
I am working on a project looking at the cytotoxic effects of lipid oxidation products on caco-2 cells. I would like to include a sample group with added antioxidants as a 'negative control'. What...
19 May 2023 1,748 2 View
I have a data set of peak areas from gas chromatography I would like to run on a PLSR model. Generally, for PLSR I would center and scale the data, is that appropriate here? As the peaks differ...
13 March 2023 6,683 1 View
As stated above is there a way to assign statistical significance (ie get a p-value) from the individual VIPs of RCs of a PLS model? There is the obvious issue that the VIP is a single point and...
08 March 2023 3,033 6 View
Does anyone have experience with using microspheres (like Thermo's FluoSpheres™) to evaluate caco-2 monolayer permeability on transwell inserts? If so do you have any references or protocols you...
28 July 2022 322 0 View
I have been working on developing an static in vitro digestion procedure with downstream application to Caco-2 cell cultures directly. When reviewing the literature many papers utilize dialysis...
20 May 2022 4,395 2 View
