Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Ideas for Discussion of a Case-study analysis?

I am currently researching the demotivating factors and remotivating factors of high school students in my country. My study only has 13 participants, and I groups them into 3 groups depending on...

02 December 2020 2,288 3 View

About cross-case analysis?

Currently, I have finished interviewing my high school students to learn about their demotivating and remotivating factors. I'm trying to use case-study method to analysis data. In my...

17 November 2020 7,989 2 View

Dear All, could you please show me how should I do to open SIR-C compressed image with ENVI 5.3 or ArcGIS 10.4 softwares?

I would like to apply a remote sensing processing in this file for a structural geologic feature study. The compressed file name: PR48120.tar The three unzipped files which come from PR48120.tar...

23 September 2020 4,924 2 View

Do you have any references on "Process to develop Child centered Social accountability"?

Dear all , I am working as the consultant for Save Children International to develop and pilot a process named " Child Centered Social accountability" in Vietnam. Does anyone know where I can get...

31 August 2020 6,297 5 View

How to obtain clear Ru 3d5/2 peaks?

I am trying to obtain Ru 3d XPS spectra of samples with RuO2 content of 0.1 ~ 0.3%. But due to the overlapping of the C 1s region and maybe the low content as well, the Ru 3d5/2 peak was almost...

22 July 2020 7,789 4 View

About researching EFL learning demotivation and Straussian grounded theory?

Currently, I am conducting a MA dissertation by using Strauss and Corbin's Grounded Theory. My topic is researching EFL learning demotivation of high school students (16 to 18 years old). My main...

13 July 2020 6,773 4 View

What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,177 1 View

Any recommendation for PhD research topic in consumer culture?

Hello everyone, I am stuck with my PhD proposal ideas. I want to focus on globalization and cosmopolitan consumers. I would be very appreciate if you can provide me some key papers and suggestion...

24 March 2020 8,227 3 View

Which Grounded Theory should I use?

I am a novice researcher who is writing my dissertation. My topic is high school students' demotivation and their coping strategies in my country. Currently, I am thinking about using Straussian...

15 February 2020 2,243 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View