Command line for results in autodock4?

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Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to use the BLAST primer to check the possible bonds of primer?

Hi everyone, I run PCR to checked presence of specific gene in Clostridium perfringens but I received 2 bands, one band showed product size expected and another one showed higher product size. I...

31 May 2024 645 1 View

Which condition storage is suitable for azide-labelled protein/peptide?

I used NHS-PEG4-Azide to label protein and temporarily store the labelled protein at 4 degrees. Is it suitable for long-term storage at this temperature?

21 April 2024 2,832 2 View

Does anyone can help me recognize what families these cephalopod paralarvae belong to?

I'm trying to identify some paralarvae in the central of Vietnam. I hope some experts can help me address this.

14 April 2024 6,425 0 View

Increase gDNA yield for mollusk tissue extraction?

I regularly have low DNA concentrations on the vetigastropod tissues I extract. I have tried fresh tissue and older/museum tissues. We use the Thermo Scientific GeneJET Genomic DNA Purification...

08 April 2024 3,611 4 View

Help for Mitochondrial 16S rRNA PCR?

Hi All, I am trying to amplify mitochondrial 16S gene for marine snails (Calliostomatidae) and other vetigastropods, but I only get primer dimers or nothing on the gel. These primers have worked...

03 April 2024 8,736 4 View

How do teachers/ lecturers around the globe use AI tools and ChatGPT in EFL classes & in EFL writing classes?

A Literature Review of How ChatGPT is Perceived and Utilized in EFL Writing Classes: Implications for Vietnam

16 February 2024 5,894 3 View

Please! Does anyone know any documents on the identification of paralarvae cephalopods in the western Pacific region?

I am trying to identify some paralarvae of cephalopods in the South China Sea.

10 January 2024 8,054 3 View

Please! Does anyone know any documents on the identification of paralarvae cephalopods in the western Pacific region?

I am trying to identify some paralarvae of cephalopods in the South China Sea.

10 January 2024 9,402 3 View

Is there any open-access Country Risk Index subsituting the ICRG index?

I am looking for country data on risk or vulnerability from 2012-2021 for my research on supply chain resilience. I have tried FM global and ND-GAIN data but these two cover specific risks only...

18 December 2023 882 0 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

JCPDS 65-7246 file please?

Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance

04 August 2024 5,613 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View