Complex wave form in ANSYS?

Is there a way to input wave speed in a complex form on ANSYS? The wave speed I need to assign a material is 325(1+0.11i) however I have been unable to do this thus far. Failing that, is there...

27 February 2021 5,969 1 View

Absorption behaviour of anechoic model?

I have modelled a unit-cell of an anechoic structure using ANSYS. The layer is silicone and has been assigned as an acoustics physics region, with an air cavity inside (the air has been modelled...

18 February 2021 4,119 1 View

How to model an anechoic layer on ANSYS?

I am a final year university student and for my honours project I am studying the effects of the size of air-filled cavities in an anechoic layer. The layer is made of silicone and has a steel...

28 January 2021 6,745 2 View

Are the radiation produced by cell towers harmful to humans?

Especially small kids who lives near a cell tower.

21 February 2020 494 3 View

Will aluminium electrodes (cathode or anode) corrode when biased in pure deionised water?

If two aluminium electrodes, in deionised water, were biased to 5V relative to one another, would we just observe electrolysis of the water? Let us assume that the water is perfectly pure, just...

11 July 2018 9,706 7 View

Why is there a difference between the natural frequencies?

I have two plates and in between the 3rd and 4th layers I have connected them with a) tie constraints and b) surface based cohesion. My question is, should the values of natural frequency be the...

24 January 2018 445 4 View

What are the advantages and disadvantages of modal analysis when compared to dynamic steady-state analysis?

Hi, Just a quick question, what are the differences between modal analysis and dynamic steady-state analysis? The model is the same, the system is undamped and the boundary conditions are the...

16 January 2018 5,630 3 View

How do I connect elements/nodes between two shell parts together on ABAQUS?

I have created both shell parts and meshed them the same (two square plates of equal dimensions) , then i have assigned the composite layers. I am unsure on how to connect the nodes/elements of...

17 November 2017 1,537 3 View

Which statistical method should be used to find a correlation between 3 variables comparing UK data to EU average?

16 March 2017 9,791 6 View

Why are my A431 epidermoid cells dying?

I recently purchase some A431 cells from ATCC and the recommended media is DMEM + 10% FBS (my media is also supplemented with GlutaMAX). After thawing and passaging some cells attach, however the...

03 October 2016 5,940 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View