Callum McKenzie

3 Questions 4 Answers 0 Followers

Questions related from Callum McKenzie

Why are my A431 epidermoid cells dying?

I recently purchase some A431 cells from ATCC and the recommended media is DMEM + 10% FBS (my media is also supplemented with GlutaMAX). After thawing and passaging some cells attach, however the...

04 October 2016 6,020 3 View

Co-transfecting plasmids - how much transfection reagent to use?

I am co-transfecting NIH3T3 cells with two plasmids (rasV12 mutant + gene of interest) for a transformation assay. My question is: how much reagent (FuGENE HD) should I use? I typically use a 3:1...

12 February 2016 6,910 5 View

NIH3T3 cells growing very slowly. Any tips?

I have been culturing these cells for around 2 weeks and have passed them twice. They never reached confluency. I am culturing them in DMEM/F12 media + 10% NCS (Sigma) + 2 mM L-Glutamine (already...

24 April 2015 4,211 3 View