Cloning fusion gene/exons?

30 November 2016 1 860 Report

hi all,

we recently identify a junction point of exon5 of gene A and 5' UTR (exon11) of gene B. we manage to proof the junction in cdna by PCR and Sanger sequencing. however when we wanted to clone this fusion protein, we have no idea where is the putative ATG and stop codon we should look at. Any advice for that?

Chun Shen Lim

Look for the ORFs that are conserved across species. Multiple sequence alignments will give you some idea about this. dN/dS (KaKs) ratio might help too.

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