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Does anyone has good protocol/reagent for transfecting U937 cells with siRNA? It is a human macrophages and looks like they are quite sensitive and float/die after, even with very low amount of...
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I tried to detect a short non-coding RNA (80nt) but taqman detection gave me very late ct (about ct 34-36) when I synthesize cDNA with specific primers. I wish to pre-amplify it before subject it...
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