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Questions related from Wei Lun Ng
Does anyone has good protocol/reagent for transfecting U937 cells with siRNA? It is a human macrophages and looks like they are quite sensitive and float/die after, even with very low amount of...
15 August 2018 5,371 3 View
hi all, we recently identify a junction point of exon5 of gene A and 5' UTR (exon11) of gene B. we manage to proof the junction in cdna by PCR and Sanger sequencing. however when we wanted to...
30 November 2016 859 1 View
I tried to detect a short non-coding RNA (80nt) but taqman detection gave me very late ct (about ct 34-36) when I synthesize cDNA with specific primers. I wish to pre-amplify it before subject it...
17 August 2016 336 4 View
does anyone has any nuclear run-on protocol can share, working on cell lines. thanks
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