Wei Lun Ng

5 Questions 18 Answers 0 Followers

Questions related from Wei Lun Ng

Transient transfection siRNA on U937 cells?

Does anyone has good protocol/reagent for transfecting U937 cells with siRNA? It is a human macrophages and looks like they are quite sensitive and float/die after, even with very low amount of...

15 August 2018 5,408 3 View

Cloning fusion gene/exons?

hi all, we recently identify a junction point of exon5 of gene A and 5' UTR (exon11) of gene B. we manage to proof the junction in cdna by PCR and Sanger sequencing. however when we wanted to...

30 November 2016 912 1 View

Anyone have good pre-amplification and RT-PCR protocol for miRNA or non-coding RNAs?

I tried to detect a short non-coding RNA (80nt) but taqman detection gave me very late ct (about ct 34-36) when I synthesize cDNA with specific primers. I wish to pre-amplify it before subject it...

17 August 2016 394 4 View

Does anyone has good nuclear run-on protocol?

does anyone has any nuclear run-on protocol can share, working on cell lines. thanks

12 January 2016 373 3 View

Does anyone have experience/nice protocol in isolating viral episomes and genomic DNA, particularly EBV?

Can anyone suggest a protocol to isolate gDNA from viral episome and downstream assay that could verify the separation?

10 March 2015 9,687 0 View