Dear users,
does anyone know a commercially available vector for cloning containing a cleavable C-term His-Tag?
https://www.sigmaaldrich.com/catalog/product/sigma/ogs2769?lang=en®ion=IN&gclid=EAIaIQobChMI-sag8YeD3wIVwhyPCh1j_AQ6EAAYAiAAEgLtIvD_BwE
I was wondering if TCEP is suitable to be used in MTT- or dichlorophenilindophenol-based assays, since other reducing agents, such as DTT, actually reduce these two compounds.
06 July 2019 2,026 1 View
By overexpressing an eukaryotic mitochondrial membrane protein in E.coli, I was wondering if I can assume the same subcellular localization in bacteria cells. Otherwise, any advice about how to...
10 November 2017 6,403 3 View
I am planning to use two plasmids harboring two sequences under the control of two different promoter, T7 and araBAD. My aim is to cotransform Ecoli strain with these two plasmids so that the...
03 April 2017 8,898 1 View
I want to compare protein sequences of proteins shared between aerobic and anaerobic organisms
08 September 2015 2,914 1 View
I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...
31 December 2014 10,005 1 View
I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.
11 December 2014 5,873 18 View
I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...
08 September 2014 1,408 4 View
I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.
05 June 2014 3,264 7 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View