06 June 2017 1 8K Report

Dear users,

   does anyone know a commercially available vector for cloning containing a cleavable C-term His-Tag?

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Does TCEP reduce MTT or DCPIP (formerly DCIP)?

I was wondering if TCEP is suitable to be used in MTT- or dichlorophenilindophenol-based assays, since other reducing agents, such as DTT, actually reduce these two compounds.

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Eukaryotic mitochondrial membrane protein expressed in E.coli. How can one assess the subcellular localization?

By overexpressing an eukaryotic mitochondrial membrane protein in E.coli, I was wondering if I can assume the same subcellular localization in bacteria cells. Otherwise, any advice about how to...

10 November 2017 6,403 3 View

Does anyone know if IPTG and arabinose can be used together for induction of expression?

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Does anyone know a data base where I can catch a list of anaerobic and/or aerobic-tolerant animal organisms?

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TreeFix problem

I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...

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Can anyone advise me books dealing with phylogenetic reconstruction, mostly in its practical aspects?

I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.

11 December 2014 5,873 18 View

Does anyone know a protocol for a native PAGE gel?

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Does anyone know of organisms that lack catalase?

I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.

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