Dear users,
does anyone know a commercially available vector for cloning containing a cleavable C-term His-Tag?
https://www.sigmaaldrich.com/catalog/product/sigma/ogs2769?lang=en®ion=IN&gclid=EAIaIQobChMI-sag8YeD3wIVwhyPCh1j_AQ6EAAYAiAAEgLtIvD_BwE
I was wondering if TCEP is suitable to be used in MTT- or dichlorophenilindophenol-based assays, since other reducing agents, such as DTT, actually reduce these two compounds.
06 July 2019 2,118 1 View
By overexpressing an eukaryotic mitochondrial membrane protein in E.coli, I was wondering if I can assume the same subcellular localization in bacteria cells. Otherwise, any advice about how to...
10 November 2017 6,473 3 View
I am planning to use two plasmids harboring two sequences under the control of two different promoter, T7 and araBAD. My aim is to cotransform Ecoli strain with these two plasmids so that the...
03 April 2017 9,020 1 View
I want to compare protein sequences of proteins shared between aerobic and anaerobic organisms
08 September 2015 3,011 1 View
I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...
31 December 2014 10,125 1 View
I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.
11 December 2014 5,948 18 View
I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...
08 September 2014 1,484 4 View
I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.
05 June 2014 3,377 7 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View