Fulvio Saccoccia

9 Questions 6 Answers 0 Followers

Questions related from Fulvio Saccoccia

Does TCEP reduce MTT or DCPIP (formerly DCIP)?

I was wondering if TCEP is suitable to be used in MTT- or dichlorophenilindophenol-based assays, since other reducing agents, such as DTT, actually reduce these two compounds.

07 July 2019 2,084 1 View

Eukaryotic mitochondrial membrane protein expressed in E.coli. How can one assess the subcellular localization?

By overexpressing an eukaryotic mitochondrial membrane protein in E.coli, I was wondering if I can assume the same subcellular localization in bacteria cells. Otherwise, any advice about how to...

11 November 2017 6,447 3 View

Cleavable C-terminal His-Tag vector for cloning

Dear users, does anyone know a commercially available vector for cloning containing a cleavable C-term His-Tag?

06 June 2017 7,675 1 View

Does anyone know if IPTG and arabinose can be used together for induction of expression?

I am planning to use two plasmids harboring two sequences under the control of two different promoter, T7 and araBAD. My aim is to cotransform Ecoli strain with these two plasmids so that the...

04 April 2017 8,990 1 View

Does anyone know a data base where I can catch a list of anaerobic and/or aerobic-tolerant animal organisms?

I want to compare protein sequences of proteins shared between aerobic and anaerobic organisms

09 September 2015 2,983 1 View

TreeFix problem

I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...

01 January 2015 10,066 1 View

Can anyone advise me books dealing with phylogenetic reconstruction, mostly in its practical aspects?

I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.

12 December 2014 5,914 18 View

Does anyone know a protocol for a native PAGE gel?

I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...

09 September 2014 1,458 4 View

Does anyone know of organisms that lack catalase?

I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.

06 June 2014 3,335 7 View