Does TCEP reduce MTT or DCPIP (formerly DCIP)?

I was wondering if TCEP is suitable to be used in MTT- or dichlorophenilindophenol-based assays, since other reducing agents, such as DTT, actually reduce these two compounds.

06 July 2019 2,026 1 View

Eukaryotic mitochondrial membrane protein expressed in E.coli. How can one assess the subcellular localization?

By overexpressing an eukaryotic mitochondrial membrane protein in E.coli, I was wondering if I can assume the same subcellular localization in bacteria cells. Otherwise, any advice about how to...

10 November 2017 6,403 3 View

Cleavable C-terminal His-Tag vector for cloning

05 June 2017 7,626 1 View

Does anyone know if IPTG and arabinose can be used together for induction of expression?

I am planning to use two plasmids harboring two sequences under the control of two different promoter, T7 and araBAD. My aim is to cotransform Ecoli strain with these two plasmids so that the...

03 April 2017 8,898 1 View

TreeFix problem

I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...

31 December 2014 10,005 1 View

Can anyone advise me books dealing with phylogenetic reconstruction, mostly in its practical aspects?

I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.

11 December 2014 5,873 18 View

Does anyone know a protocol for a native PAGE gel?

I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...

08 September 2014 1,408 4 View

Does anyone know of organisms that lack catalase?

I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.

05 June 2014 3,264 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View