Hi, I'm wondering what ways you use for improving the clearing of conjugate from nitrocellulose. my device has to use a large amount of gold conjugate and at the end, my NC is left with a noticeable faint purple color. I would like for my NC to be completely white at the end, any ideas?
I already used triton-x100, tween 20 and some other surfactants, but they don't do much.
Obviously blocking is an option but from experience i can say that the improvement is minimal and the work associated to it is not worth it.
This is a problem of background signal on the nitrocellulose but the test line is working fine, I just need to clear the excess conjugate from the surface. Any tips or tricks are appreciated, thank you
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