Clean DNA samples with dialysis?

10 October 2016 1 5K Report

Hi all,

I heard it is possible to clean the own DNA sample (after extraction) by performing a sort of "dialysis "with a floating filter disc in H2O...Can you explain me how it works and how this procedure is carried out?I did not understand it.

Thanks

Kearstin Edmonds

Hello, I see your question was a year ago so you may not need this anymore! However, there is a method of drop dialysis that can be found here.

https://www.neb.com/protocols/2013/09/16/drop-dialysis

It functions to gently clean out salts and other substances by allowing the sample to filter osmotically through small pores on the membrane.

Incubation is usually at room temperature and times can vary based off of what you're purifying.

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