Kearstin Edmonds

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Questions related from Kearstin Edmonds

Electroporation of Plasmid DNA and electrocompetent E. coli DH5a, how much to use of each?

I am doing an electroporation of Plasmid DNA (~1kbp) and electrocompetent E. coli DH5a. How many uL of cells and DNA should I use. I have 1uL DNA and 25uL cells per NEB protocol but I have found...

11 November 2017 9,665 5 View

Does BioMix contain anything special that cannot be gained from a DIY master mix?

My protocol calls for BioMix by Bioline. However, my project has a budget. Does anyone know if the reagents I use for Vent PCR would suffice as a replacement for BioMix?

01 January 1970 2,947 1 View