Working on purifying a DNA binding enzyme, pI in the range of 9. Losing it in flow-through on cation exchange, considering another type of affinity column, either Cibacron Blue or Heparin column to eliminate bound DNA.
Wondering if anyone has experience with or can comment on relative efficiency of these two media for purifying DNA binding proteins? Greater success with one vs the other? Comments and suggestions appreciated.
Besides heparin, hydroxyapatite may be a good choice to try. I guess that is a natural protein (not recombinant) son His-tag is not an option.
Heparin is a good option. This works well for DNA binding proteins.
I have used a library of 96 cibacron blue based affinity dyes. I guess your target DNA may bind with one of the dye. These psedo affinity dyes and you need to screen first. Let me know if you need the full text or if you have any other query.Article Discriminatory protein binding by a library of 96 new affini...
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