Kirk Hevener

4 Questions 4 Answers 0 Followers

Questions related from Kirk Hevener

Are there any other "innocuous" proteins (other than BSA) that can be added to an assay buffer conditions to stabilize an enzyme?

We are working with a reductase enzyme that is very unstable at dilute concentrations (functional dimer, likely dissociating when dilute). We've explored increasing salt and buffer...

06 June 2018 6,577 2 View

How to extend the activity of a dimer enzyme in a biochemical asay?

The enzyme is a functional dimer that is losing activity rapidly when diluted to low concentrations (e.g. 50 nM). We believe this is due to dissociation. We have optimized the buffer and pH...

01 January 2018 482 3 View

Cibacron Blue versus Heparin for DNA-binding protein purification. Any advantages of one over the other?

Working on purifying a DNA binding enzyme, pI in the range of 9. Losing it in flow-through on cation exchange, considering another type of affinity column, either Cibacron Blue or Heparin column...

01 January 2014 3,951 3 View

Can use of a His8-tag (or His10) vs a His6-tag improve protein solubility in difficult cases? If so, to what extent?

Attempting to improve a target protein's solubility in recombinant expression. Currently His6 tagged and experiencing both low yield (inclusion bodies), and poorly soluble product (difficulty...

08 August 2013 3,789 4 View