May I ask a question.. I did calibration curve for chrome using concentrations of 20, 40, 60, 80, and 100 mg/l of chromium.
After that I added diphenylcarbazide and phosphoric acid on chrome solution and it gave me purple color.
When I was measuring it on the spectrophotometer
I noticed that the color of the two flasks that have a concentration of 80 and 100 mg/l disappeared after ten minutes.
can I know why?