I am currently planning an experiment that requires magnetic bead sorting of ductal cells from a single cell suspension of the mouse pancreas. My goal is to sort out ductal cells from the single cell suspension.

The mouse being utilized has a dual-color fluorescent reporter (mT/mG), so I want to avoid FITC and TRITC conjugated primary antibodies. I plan to use a DBA lectin antibody to target the ductal population.

I see that there are many different types of DBA conjugated antibodies outside of FITC and TRITC. I was initially thinking DBA-Cy5 but also noticed there are DBA-Biotin and DBA-rhodamine conjugated antibodies.

I plan to use anti-Cy5, Biotin, or Rhodamine conjugated microbeads but was unsure if there was any advantage to one over the other?

Any suggestions would be appreciated!

Thanks!

Kyle