Kyle McAndrews

6 Questions 3 Answers 0 Followers

Questions related from Kyle McAndrews

[4OHT] for CreERT2 activation in pancreatic mouse organoids?

Hello, I am wandering if anyone has a recommendation for the proper concentration of 4-hydroxytamoxifen to induce CreERT2 expression in primary pancreatic ductal organoids generated from...

27 February 2021 5,897 1 View

Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

24 December 2020 4,872 1 View

Automatic cell counting? (Ductal epithelium cells with fluorescence)?

Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...

21 September 2020 2,029 6 View

Left tissue in 4% PFA O/N accidentally, do I need to do antigen retrieval?

I accidentally left my tissue (mouse pancreas/duodenum/lung/spleen) in PFA overnight instead of 30% sucrose. Typically, I fix the tissue in 4% PFA for 45-60 minutes and then 30% sucrose O/N. I...

07 July 2020 4,195 0 View

Does storing cryosections at room temperature effect DNA methylation analysis?

Hello, I am planning on doing DNA methylation analysis on frozen mouse pancreatic tissue using laser capture microdissected samples. I have many slides from snap-frozen tissue embedded in OCT...

11 April 2019 569 2 View

Standard PCR of Brainbow 2.1 and mT/mG mice (overlap)?

Hello, I have recently begun to introduce the Brainbow 2.1 gene to some of our inducible Cre mice. We have used the mT/mG reporter in the past with success. I recently had some extra master mix...

25 September 2018 4,465 1 View