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Questions related from Kyle McAndrews
Hello, I have a planned experiment using organoids derived from a Rosa26mT/mG (dual color, Cre inducible reporter) PDAC GEMM. On my first pilot study all of the immune competent mice have seemed...
05 November 2022 1,581 0 View
I have a planned experiment that involves the following 1) MACs sorting of specific primary cells (single cell suspension) from an inducible Cre mouse which is tamoxifen naive. 2) Grow these...
07 April 2022 1,814 0 View
Hello, I am wandering if anyone has a recommendation for the proper concentration of 4-hydroxytamoxifen to induce CreERT2 expression in primary pancreatic ductal organoids generated from...
27 February 2021 5,906 1 View
Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...
24 December 2020 4,900 1 View
Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...
21 September 2020 2,105 6 View
I accidentally left my tissue (mouse pancreas/duodenum/lung/spleen) in PFA overnight instead of 30% sucrose. Typically, I fix the tissue in 4% PFA for 45-60 minutes and then 30% sucrose O/N. I...
07 July 2020 4,209 0 View
Hello, I am planning on doing DNA methylation analysis on frozen mouse pancreatic tissue using laser capture microdissected samples. I have many slides from snap-frozen tissue embedded in OCT...
11 April 2019 585 2 View
Hello, I have recently begun to introduce the Brainbow 2.1 gene to some of our inducible Cre mice. We have used the mT/mG reporter in the past with success. I recently had some extra master mix...
25 September 2018 4,483 1 View
