Can anyone recommend a method for ethylene gas detection in an air sample?

I am looking for a method to detect ethylene gas concentration in a green house. I have tried SPME , Head space and GC-MS analysis but results were not satisfied. So is it better to detect...

10 January 2019 7,832 3 View

Contamination in A549 cell culture?

I am culturing A549 cells, I have noticed there are white color particle like things floating on the media. it was visible to the naked eye also. there was no color change in the media or there...

22 February 2018 8,404 5 View

Why there were many floating cell, when THP-1 cells treated with PMA ?

I have done THP-1 cell differentiation into macrophages. I used RPMI as a media. and PMA 30nM concentration. after 24 hours incubation there were many floating cells. what could be the reason? and...

22 January 2018 3,473 3 View

Could anyone explain me the difference between non-verbal mental age and verbal mental age please?

14 March 2017 8,969 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Do you consider that rose bengal chloramphenicol agar is suitable to isolate and count fungus?

Someone knows about a specific law or regulation which mentions the use of the rose bengal chloramphenicol agar is suitable to isolate and count fungi. Perhaps, someone has done a research about...

07 January 2021 5,325 5 View

Gibson cloning Transformation troubleshooting. How to make it work?

My transformation after Gibson cloning isn't working. I am using NEB-alpha cells which are good for transformation but even positive control that comes with Gibson kit didn't work on the...

01 February 2020 8,517 4 View

Is it possible to get bacterial contamination in DNA extractions?

I have been getting contaminated plates and preps with what seems to be a gram negative bacteria (Acinetobacter pittii according to Blast) that is resistant to Chloramphenicol. I'm trying to...

11 December 2019 6,397 7 View

How to find the downstream of a histone acetyltransferase gene?

Hi all, I am working on the interaction between C. elegans and the nematode-trapping fungus. Now I found a gene in C .elegans called epc-1 which will have the phenotype I want when it was...

10 November 2019 2,677 4 View

How to optimize vector backbone to have higher plasmid yields?

Hi, I am trying to optimize plasmid yield to prepare for animal experiments. So far, I can only get about 0.6~0.7mg from 1L starting culture. I used the Zymo Max prep kit, and I don't think that...

10 November 2019 6,894 1 View

How to optimize vector backbone to have higher plasmid yields?

Hi, I am trying to optimize plasmid yield to prepare for animal experiments. So far, I can only get about 0.6~0.7mg from 1L starting culture. I used the Zymo Max prep kit, and I don't think that...

31 October 2019 7,907 2 View

Can you help me identify mold on agar plates?

Hi guys! I could use Reddit's help in visually identifying the species of the mold growing on the three agar plates in the attached picture. The plates are Sabouraud's Dextrose Agar +...

22 July 2019 2,170 1 View

Can you help me identify mold on agar plates?

Hi guys! I could use Reddit's help in visually identifying the species of the mold growing on the three agar plates in the attached picture. The plates are Sabouraud's Dextrose Agar +...

06 July 2019 626 1 View

LB + chloramphenicol + spectinomycin selection ?

05 June 2019 4,688 2 View