Has anyone tried this assay with E.Coli cells ? I would like to know the ideas and experiences . Thank you.
Article A Rapid, Sensitive, and Inexpensive Assay for Chloramphenico...
In this study I wanted to find out what factors that can be change milk yield of dairy farmers and do farmer’s attitudes, skills on farming, knowledge on farming relate with yield of milk...
09 August 2023 5,811 5 View
We are designing a clinical trial with fibre supplementation for humans. Is there anyone with expertise in that area to be involved as a collaborator? Someone with a nutrition background and...
23 February 2023 9,555 5 View
How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate? Most of the papers referred to do not mention a volume or ratio. Only one paper...
27 August 2022 626 0 View
I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2%...
25 August 2022 3,809 3 View
For THP-1 differentiation we use reconstituted PMA at 1mg/ml. Our final concentration added to cells is 50ng/ml. Since final working concentration & volume required is low, we usually make...
03 March 2022 4,638 3 View
Has anyone had experience in growing P. gingavalis in BHI agar plates? There’s an unusual amount of water condensation inside the plate after 4 days of incubation in an anaerobic chamber, and...
13 November 2021 8,454 4 View
I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using...
01 September 2021 5,219 2 View
Is there a database for finding information about microbes in addition to NCBI? Something similar to Human Oral Microbiome Database for oral microbes. Any help is appreciated. Thank you.
04 August 2021 7,872 3 View
What are the commonly available primer sequences for the identification of S godonni and P gingavalis?
18 May 2021 6,248 2 View
What are the most commonly used types of anaerobic chambers to grow anaerobic microbes? Thank you in advance.
17 May 2021 4,488 4 View
Hello, I am currently doing a method optimization for chloramphenicol using our lab's tandem mass spec. I have done Q1 scan (prec ion: 320.10 m/z) and did MRM scan for three product ion. Upon...
24 April 2024 250 0 View
Hi All, My question is clear, so why other antibiotics and antifugal could be used, why chloramphenicol and Cycloheximide exactly? I don't want routine answers, isolating, inhibiting, no...
11 February 2024 7,090 3 View
I inoculated 20 mL of Rosetta pre-culture into 1L LB broth supplemented with 1 mL of Kanamycin and 1 mL of Chloramphenicol. After one hour of incubation, I checked the OD, and the value observed...
13 December 2023 4,927 1 View
It was found that methanolic extract showed synergistic effect with amoxyclav (against K. pneumonia, B. subtilis, St. aureus), chloramphenicol (against K. pneumonia and St. aureus), erythromycin...
23 November 2023 361 4 View
Hi everyone, I am currently working on protein expression and purification. Transformation was successful using Lemo21 E.coli and plasmid pRSETA. Single colony was inoculated into 100mL LB broth...
18 November 2023 137 6 View
I observed an increment in the absorbance of chloramphenicol using a UV spectrophotometer.
13 December 2022 7,341 1 View
I use the following composition for 5o ml NK cells culture media (alpha MEM) : 12.5 % Horse serum, 12.5% FBS, 2mM L-glutamine, 0.2mM-inositol, 20µM Folic acid 10 ^ (-4) M β-Mercapto Ethanol, 0.2%...
04 September 2022 6,554 7 View
Hi, I have recently attempted natural competence transformation with Bacillus subtilis (WB800N), following the protocol from Mobitec. I used 5ug/ml chloramphenicol for my selection. My...
24 June 2022 9,128 0 View
In our laboratory, while isolating disease-causing fungal phytopathogens; we faced immense troubles due to contamination. Bacterial contamination were inhibited by using various antibacterial such...
16 April 2022 8,796 6 View
Hi everyone ! I'm trying to mutate Bacmid (MultiBac™ - Geneva Biotech) by using lambda red recombination. I've Knocked out particular gene in bacmid and replaced with cat-SacB cassette....
15 December 2021 4,853 4 View